GSTP1 Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications
Joint Authors
Salvi, Samanta
Gurioli, Giorgia
Martignano, Filippo
Foca, Flavia
De Giorgi, Ugo
Calistri, Daniele
Costantini, Matteo
Gunelli, Roberta
Casadio, Valentina
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
GSTP1 belongs to the GSTs family, a group of enzymes involved in detoxification of exogenous substances and it also plays an important role in cell cycle regulation.
Its dysregulation correlates with a large variety of tumors, in particular with prostate cancer.
We investigated GSTP1 methylation status with methylation specific PCR (MS-PCR) in prostate cancer (PCa) and in benign tissue of 56 prostatectomies.
We also performed immunohistochemistry (IHC) so as to correlate gene methylation with gene silencing.
GSTP1 appears methylated in PCa and not in healthy tissue; IHC confirmed that methylation leads to protein underexpression ( p < 0.001 ).
GSTP1 is highly expressed in basal cell layer and luminal cells in benign glands while in prostatic intraepithelial neoplasia (PIN) it stains only basal cell layer, whereas PCa glands are completely negative.
We demonstrated that methylation leads to underexpression of GSTP1.
The progressive loss of GSTP1 expression from healthy glands to PIN and to PCa glands underlines its involvement in early carcinogenesis.
American Psychological Association (APA)
Martignano, Filippo& Gurioli, Giorgia& Salvi, Samanta& Calistri, Daniele& Costantini, Matteo& Gunelli, Roberta…[et al.]. 2016. GSTP1 Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications. Disease Markers،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103705
Modern Language Association (MLA)
Martignano, Filippo…[et al.]. GSTP1 Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications. Disease Markers No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1103705
American Medical Association (AMA)
Martignano, Filippo& Gurioli, Giorgia& Salvi, Samanta& Calistri, Daniele& Costantini, Matteo& Gunelli, Roberta…[et al.]. GSTP1 Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103705