Human Hemochromatosis Protein (HFE) Immunoperoxidase Stain Highlights Choriocarcinoma within Mixed Germ Cell Tumors
Joint Authors
Talmon, Geoffrey
Cox, Jesse L.
Koepsell, Scott A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Identification of choriocarcinoma within a germ cell tumor can have major implications for the subsequent staging and treatment of testicular neoplasms.
Immunoperoxidase staining greatly enhances the speed and sensitivity of identifying occult, though clinically significant, tumor components.
In mixed germ cell tumors, staining for beta-human chorionic gonadotropin (β-hCG) has been historically used to assess for the presence and burden of choriocarcinoma.
However, current β-hCG stains produce variable, intense staining of trophoblastic elements and surrounding tissues, clouding the assessment of true-positive staining.
Human hemochromatosis protein (HFE) is a membrane bound mediator of iron transport expressed at high levels within placenta.
Additionally, previous reports have demonstrated that choriocarcinoma cell lines express HFE, although in vivo expression had not been examined.
To address whether HFE can stain trophoblastic elements, HFE immunohistochemistry was conducted in choriocarcinoma (n=4), mixed germ cell tumors (n=11), seminoma (n=4), and placenta (n=11).
HFE consistently demonstrated cytoplasmic and membranous staining, highlighting both syncytiotrophoblasts and cytotrophoblasts within choriocarcinoma and placenta.
Staining of intratumoral white blood cells was observed within seminomas and mixed germ cell tumors, corroborating prior reports stating that HFE highlights monocytes and macrophages.
Taken together, HFE may serve as an alternative target from β-hCG for immunoperoxidase studies when highlighting choriocarcinoma.
American Psychological Association (APA)
Cox, Jesse L.& Talmon, Geoffrey& Koepsell, Scott A.. 2016. Human Hemochromatosis Protein (HFE) Immunoperoxidase Stain Highlights Choriocarcinoma within Mixed Germ Cell Tumors. Disease Markers،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103722
Modern Language Association (MLA)
Cox, Jesse L.…[et al.]. Human Hemochromatosis Protein (HFE) Immunoperoxidase Stain Highlights Choriocarcinoma within Mixed Germ Cell Tumors. Disease Markers No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1103722
American Medical Association (AMA)
Cox, Jesse L.& Talmon, Geoffrey& Koepsell, Scott A.. Human Hemochromatosis Protein (HFE) Immunoperoxidase Stain Highlights Choriocarcinoma within Mixed Germ Cell Tumors. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1103722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103722