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Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer
Joint Authors
Li, Wen-han
Zhou, Zhang-jian
Huang, Tian-he
Guo, Kun
Chen, Wei
Wang, Ying
Zhang, Hao
Song, Yong-chun
Chang, Dong-min
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
This study was to evaluate the diagnostic value of OSR2, VAV3, and PPFIA3 hypermethylation in gastric cancer (GC) patients.
Patients and Methods.
By using methylation-specific polymerase chain reaction (MSP), we detected the methylation status in tissue and serum samples from 48 gastric cancer (GC) patients and 25 normal individuals.
Results.
We found that OSR2, VAV3, and PPFIA3 were methylated in 70.8% (34/48), 54.2% (26/48), and 60.4% (29/48) of GC tissue, respectively.
On the contrary, those genes were barely methylated in their paired paracancerous histological normal tissues (PCHNTs) (all P values < 0.01).
We next analyzed the methylated OSR2, VAV3, and PPFIA3 in serum DNA.
Compared with 25 normal individuals, those three genes were significantly hypermethylated in GC patients serum samples (all P values < 0.01).
Regarding their diagnostic value in serum samples, the combined sensitivity of at least one positive among the three markers in serum was 83.3%, with a specificity of 88%.
Conclusion.
Our test suggested that methylation of OSR2, VAV3, and PPFIA3 genes in serum sample may offer a good alternative in a simple, promising, and noninvasive detection of GC.
American Psychological Association (APA)
Li, Wen-han& Zhou, Zhang-jian& Huang, Tian-he& Guo, Kun& Chen, Wei& Wang, Ying…[et al.]. 2016. Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer. Disease Markers،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103731
Modern Language Association (MLA)
Li, Wen-han…[et al.]. Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer. Disease Markers No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1103731
American Medical Association (AMA)
Li, Wen-han& Zhou, Zhang-jian& Huang, Tian-he& Guo, Kun& Chen, Wei& Wang, Ying…[et al.]. Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103731