1H NMR-Based Analysis of Serum Metabolites in Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats
Joint Authors
Lin, Taijie
Gu, Jinping
Huang, Caihua
Zheng, Suli
Lin, Xu
Xie, Liangdi
Lin, Donghai
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aims.
To study the changes of the metabolic profile during the pathogenesis in monocrotaline (MCT) induced pulmonary arterial hypertension (PAH).
Methods.
Forty male Sprague-Dawley (SD) rats were randomly divided into 5 groups ( n = 8 , each).
PAH rats were induced by a single dose intraperitoneal injection of 60 mg/kg MCT, while 8 rats given intraperitoneal injection of 1 ml normal saline and scarified in the same day (W0) served as control.
Mean pulmonary arterial pressure (mPAP) was measured through catherization.
The degree of right ventricular hypertrophy and pulmonary hyperplasia were determined at the end of first to fourth weeks; nuclear magnetic resonance (NMR) spectra of sera were then acquired for the analysis of metabolites.
Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to discriminate different metabolic profiles.
Results.
The prominent changes of metabolic profiles were seen during these four weeks.
Twenty specific metabolites were identified, which were mainly involved in lipid metabolism, glycolysis, energy metabolism, ketogenesis, and methionine metabolism.
Profiles of correlation between these metabolites in each stage changed markedly, especially in the fourth week.
Highly activated methionine and betaine metabolism pathways were selected by the pathway enrichment analysis.
Conclusions.
Metabolic dysfunction is involved in the development and progression of PAH.
American Psychological Association (APA)
Lin, Taijie& Gu, Jinping& Huang, Caihua& Zheng, Suli& Lin, Xu& Xie, Liangdi…[et al.]. 2016. 1H NMR-Based Analysis of Serum Metabolites in Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Disease Markers،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1103733
Modern Language Association (MLA)
Lin, Taijie…[et al.]. 1H NMR-Based Analysis of Serum Metabolites in Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Disease Markers No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1103733
American Medical Association (AMA)
Lin, Taijie& Gu, Jinping& Huang, Caihua& Zheng, Suli& Lin, Xu& Xie, Liangdi…[et al.]. 1H NMR-Based Analysis of Serum Metabolites in Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1103733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103733