Analysis of Urinary Prostate-Specific Antigen Glycoforms in Samples of Prostate Cancer and Benign Prostate Hyperplasia
Joint Authors
Chen, Chein-Hung
Tzai, Tzong-Shin
Hsiao, Chun-Jen
Yang, Wen-Horng
Chen, Chung-Hsuan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Glycans of prostate-specific antigen (PSA) in prostate cancer were found to be different from that in benign disease.
It is difficult to analyze heterogeneous PSA glycoforms in each individual specimen because of low protein abundance and the limitation of detection sensitivity.
We developed a method for prostate cancer diagnosis based on PSA glycoforms.
Specific glycoforms were screened in each clinical sample based on liquid chromatography-tandem mass spectrometry with ion accumulation.
To look for potential biomarkers, normalized abundance of each glycoform in benign prostate hyperplasia (BPH) and in prostate cancer was evaluated.
The PSA glycoform, Hex5HexNAc4NeuAc1dHex1, and monosialylated, sialylated, and unfucosylated glycoforms differed significantly between the prostate cancer and BPH samples.
The detection sensitivity (87.5%) and specificity (60%) for prostate cancer identification are higher than those of the serum PSA marker.
As low as 100 amol PSA could be detected with the ion accumulation method which has not been reported before.
The improved detection specificity can help reduce unnecessary examinations.
American Psychological Association (APA)
Hsiao, Chun-Jen& Tzai, Tzong-Shin& Chen, Chein-Hung& Yang, Wen-Horng& Chen, Chung-Hsuan. 2016. Analysis of Urinary Prostate-Specific Antigen Glycoforms in Samples of Prostate Cancer and Benign Prostate Hyperplasia. Disease Markers،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103799
Modern Language Association (MLA)
Hsiao, Chun-Jen…[et al.]. Analysis of Urinary Prostate-Specific Antigen Glycoforms in Samples of Prostate Cancer and Benign Prostate Hyperplasia. Disease Markers No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1103799
American Medical Association (AMA)
Hsiao, Chun-Jen& Tzai, Tzong-Shin& Chen, Chein-Hung& Yang, Wen-Horng& Chen, Chung-Hsuan. Analysis of Urinary Prostate-Specific Antigen Glycoforms in Samples of Prostate Cancer and Benign Prostate Hyperplasia. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103799