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Evaluation of the Isoflavone Genistein as Reversible Human Monoamine Oxidase-A and -B Inhibitor
Joint Authors
Elshami, Faisel
Messeha, Samia S.
Soliman, Karam F. A.
Zarmouh, Najla O.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Monoamine oxidases inhibitors (MAOIs) are effective therapeutic drugs for managing Parkinson’s disease (PD) and depression.
However, their irreversibility may lead to rare but serious side effects.
As finding safer and reversible MAOIs is our target, we characterized the recombinant human (h) MAO-A and MAO-B inhibition potentials of two common natural isoflavones, genistein (GST) and daidzein (DZ) using luminescence assay.
The results obtained showed that DZ exhibits partial to no inhibition of the isozymes examined while GST inhibited hMAO-B (IC50 of 6.81 μM), and its hMAO-A inhibition was more potent than the standard deprenyl.
Furthermore, the reversibility, mode of inhibition kinetics, and tyramine oxidation of GST were examined.
GST was a time-independent reversible and competitive hMAO-A and hMAO-B inhibitor with a lower K i of hMAO-B (1.45 μM) than hMAO-A (4.31 μM).
GST also inhibited hMAO-B tyramine oxidation and hydrogen peroxide production more than hMAO-A.
Docking studies conducted indicated that the GST reversibility and hMAO-B selectivity of inhibition may relate to C5-OH effects on its orientation and its interactions with the threonine 201 residue of the active site.
It was concluded from this study that the natural product GST has competitive and reversible MAOs inhibitions and may be recommended for further investigations as a useful therapeutic agent for Parkinson’s disease.
American Psychological Association (APA)
Zarmouh, Najla O.& Messeha, Samia S.& Elshami, Faisel& Soliman, Karam F. A.. 2016. Evaluation of the Isoflavone Genistein as Reversible Human Monoamine Oxidase-A and -B Inhibitor. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103903
Modern Language Association (MLA)
Zarmouh, Najla O.…[et al.]. Evaluation of the Isoflavone Genistein as Reversible Human Monoamine Oxidase-A and -B Inhibitor. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1103903
American Medical Association (AMA)
Zarmouh, Najla O.& Messeha, Samia S.& Elshami, Faisel& Soliman, Karam F. A.. Evaluation of the Isoflavone Genistein as Reversible Human Monoamine Oxidase-A and -B Inhibitor. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103903
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103903