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Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the OpioidNitric Oxide-Mediated, but cGMP-Independent, Pathways
Joint Authors
Zakaria, Zainul Amiruddin
Sani, M. H. Mohd.
Omar, Maizatul Hasyima
Ahmad, Zuraini
Abdul Rahim, Mohammad Hafiz
Cheema, Manraj Singh
Ching, Siew Mooi
Abdul Kadir, Arifah
Yakob, Yusnita
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The objectives of the present study were to determine the mechanisms of antinociceptive effect of methanol extract of Clinacanthus nutans (Acanthaceae) leaves (MECN) using various animal nociceptive models.
The antinociceptive activity of orally administered 10% DMSO, 100 mg/kg acetylsalicylic acid (ASA), 5 mg/kg morphine, or MECN (100, 250, and 500 mg/kg) was determined using the acetic acid-induced abdominal constriction (ACT), formalin-induced paw licking (FT), and hot plate tests (HPT).
The role of opioid and nitric oxide/cyclic guanosine monophosphate (NO/cGMP) systems was also investigated.
The results showed that MECN produced a significant (p<0.05) antinociceptive response in all nociceptive models with the recorded ED50 value of 279.3 mg/kg for the ACT, while, for the early and late phases of the FT, the value was >500 mg/kg or 227.7 mg/kg, respectively.
This antinociceptive activity was fully antagonized by naloxone (a nonselective opioid antagonist) but was partially reversed by l-arginine (l-arg; a nitric oxide [NO] precursor), Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; an NO synthase inhibitor), or their combinations thereof.
In contrast, 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ; a soluble guanylyl cyclase inhibitor) enhanced the extract’s antinociception.
UHPLC analysis revealed the presence of several flavonoid-based compounds with antinociceptive action.
In conclusion, MECN exerted the peripherally and centrally mediated antinociceptive activity via the modulation of the opioid/NO-mediated, but cGMP-independent, systems.
American Psychological Association (APA)
Abdul Rahim, Mohammad Hafiz& Zakaria, Zainul Amiruddin& Sani, M. H. Mohd.& Omar, Maizatul Hasyima& Yakob, Yusnita& Cheema, Manraj Singh…[et al.]. 2016. Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the OpioidNitric Oxide-Mediated, but cGMP-Independent, Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1103907
Modern Language Association (MLA)
Abdul Rahim, Mohammad Hafiz…[et al.]. Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the OpioidNitric Oxide-Mediated, but cGMP-Independent, Pathways. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1103907
American Medical Association (AMA)
Abdul Rahim, Mohammad Hafiz& Zakaria, Zainul Amiruddin& Sani, M. H. Mohd.& Omar, Maizatul Hasyima& Yakob, Yusnita& Cheema, Manraj Singh…[et al.]. Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the OpioidNitric Oxide-Mediated, but cGMP-Independent, Pathways. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1103907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103907