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Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima
Joint Authors
Abd El Baky, Hanaa H.
El Baroty, Gamal S.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
L-asparaginase (L-AsnA) is widely distributed among microorganisms and has important applications in medicine and in food technology sectors.
Therefore, the ability of the production, purification, and characterization of AsnA from Spirulina maxima (SM) were tested.
SM cultures grown in Zarrouk medium containing different N2 (in NaNO3 form) concentrations (1.25, 2.50, and 5.0 g/L) for 18 days contained a significant various quantity of dry biomass yields and AsnA enzyme levels.
MS L-AsnA activity was found to be directly proportional to the N2 concentration.
The cultures of SM at large scales (300 L medium, 5 g/L N2) showed a high AsnA enzyme activity (898 IU), total protein (405 mg/g), specific enzyme activity (2.21 IU/mg protein), and enzyme yield (51.28 IU/L) compared with those in low N2 cultures.
The partial purification of crude MS AsnA enzyme achieved by 80% ammonium sulfate AS precipitated and CM-Sephadex C-200 gel filtration led to increases in the purification of enzyme with 5.28 and 10.91 times as great as that in SM crude enzymes.
Optimum pH and temperature of purified AsnA for the hydrolyzate were 8.5 and 37 ± 0.2°C, respectively.
To the best of our knowledge, this is the first report on L-asparaginase production in S.
maxima.
American Psychological Association (APA)
Abd El Baky, Hanaa H.& El Baroty, Gamal S.. 2016. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103927
Modern Language Association (MLA)
Abd El Baky, Hanaa H.& El Baroty, Gamal S.. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1103927
American Medical Association (AMA)
Abd El Baky, Hanaa H.& El Baroty, Gamal S.. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103927
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103927