In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte
Joint Authors
Kengkoom, Kanchana
Ampawong, Sumate
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Phikud Navakot (PN), Thai herbal remedy in National List of Essential Medicines, has been claimed to reduce many cardiovascular symptoms especially dizziness and fainting.
Apart from blood supply, erythrocyte morphology, in both shape and size, is one of the main consideration factors in cardiovascular diseases and may be affected by vascular oxidative stress.
However, little is known about antioxidative property of PN on erythrocyte to preserve red blood cell integrity.
In this study, 1,000 μM hydrogen peroxide-induced oxidative stress was conducted on sheep erythrocyte.
Three doses of PN (1, 0.5, and 0.25 mg/mL) and 10 μM of ascorbic acid were compared.
The released hemoglobin absorbance was measured to demonstrate hemolysis.
Electron microscopic and immunohistochemical studies were also performed to characterize dysmorphic erythrocyte and osmotic ability in relation to aquaporin- (AQP-) 1 expression, respectively.
The results revealed that all doses of PN and ascorbic acid decreased the severity of dysmorphic erythrocyte, particularly echinocyte, acanthocyte, knizocyte, codocyte, clumping, and other malformations.
However, the most effective was 0.5 mg/mL PN dosage.
In addition, hydrostatic pressure may be increased in dysmorphic erythrocyte in association with AQP-1 upregulation.
Our results demonstrated that PN composes antioxidative effect to maintain the integrity and osmotic ability on sheep erythrocyte.
American Psychological Association (APA)
Kengkoom, Kanchana& Ampawong, Sumate. 2016. In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103946
Modern Language Association (MLA)
Kengkoom, Kanchana& Ampawong, Sumate. In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1103946
American Medical Association (AMA)
Kengkoom, Kanchana& Ampawong, Sumate. In Vitro Protective Effect of Phikud Navakot Extraction on Erythrocyte. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103946
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103946