Regulation of MAPKs Signaling Contributes to the Growth Inhibition of 1,7-Dihydroxy-3,4-dimethoxyxanthone on Multidrug Resistance A549Taxol Cells
Joint Authors
Zuo, Jian
Jiang, Hui
Zhu, Yan-Hong
Wang, Ya-Qin
Zhang, Wen
Luan, Jia-Jie
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
1,7-Dihydroxy-3,4-dimethoxyxanthone (XAN) is a bioactive compound isolated from Securidaca inappendiculata Hassk.
and validated with antiproliferative activities on a panel of cancer cell lines.
This study was designed to investigate its growth inhibitory effects on multidrug resistance (MDR) non-small cell lung carcinoma (NSCLC) cell line A549/Taxol and explore the possible linkage between modulation of MAPKs and the bioactivities.
Its growth inhibitory potency on the cells was estimated by MTT assay, and flow cytometric analysis was employed to investigate its potential cell cycle arrest and proapoptosis effects.
Expressions of hallmark proteins were assessed by Western-Blot method.
The results showed A549/Taxol cells were sensitive to XAN.
XAN inhibited the proliferation of A549/Taxol cells in the time and concentration dependent manners.
It acted as a potent inducer of apoptosis and cell cycle arrest in the cells.
Western-Blot investigation validated the proapoptosis and cell cycle arrest activities of XAN and the potential of MDR reversion.
Upregulation of p38 by XAN, which accounted for the cell cycle arrest at G2 phase, and the downregulation of ERK associated with the proapoptosis activity were also revealed.
Further analysis found p53 may be the central role mediated the bioactivities of MAPKs in A549/Taxol cells.
Based on these evidences, a conclusion has been deduced that XAN could be a potential agent for MDR NSCLC therapy targeting specifically MAPKs.
American Psychological Association (APA)
Zuo, Jian& Jiang, Hui& Zhu, Yan-Hong& Wang, Ya-Qin& Zhang, Wen& Luan, Jia-Jie. 2016. Regulation of MAPKs Signaling Contributes to the Growth Inhibition of 1,7-Dihydroxy-3,4-dimethoxyxanthone on Multidrug Resistance A549Taxol Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1103951
Modern Language Association (MLA)
Zuo, Jian…[et al.]. Regulation of MAPKs Signaling Contributes to the Growth Inhibition of 1,7-Dihydroxy-3,4-dimethoxyxanthone on Multidrug Resistance A549Taxol Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1103951
American Medical Association (AMA)
Zuo, Jian& Jiang, Hui& Zhu, Yan-Hong& Wang, Ya-Qin& Zhang, Wen& Luan, Jia-Jie. Regulation of MAPKs Signaling Contributes to the Growth Inhibition of 1,7-Dihydroxy-3,4-dimethoxyxanthone on Multidrug Resistance A549Taxol Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1103951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103951