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Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage
Joint Authors
Latip, Jalifah
Leong, Lek Mun
Chan, Kok Meng
Hamid, Asmah
Rajab, Nor Fadilah
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The use of herbal formulations has gained scientific interest, particularly in cancer treatment.
In this study, the herbal formulation of interest, denoted as C168, is a mixture of eight genera of plants.
This study aims to investigate the antiproliferative effect of C168 methanol extract (CME) on various cancer cells and its underlying mechanism of action on the most responsive cell line, namely, HCT 116 cells.
CME exerted antiproliferative activities on HCT 116 colorectal carcinoma cells and HepG2 hepatocellular carcinoma cells but not on CCD-841-CoN normal colon epithelial cells, Jurkat E6.1 lymphoblastic leukemic cells, and V79-4 Chinese hamster lung fibroblasts.
Further investigation on HCT 116 cells showed that CME induced G2/M cell-cycle arrest and apoptosis.
Treatment of CME induced oxidative stress in HCT 116 cells by increasing the superoxide anion level and decreasing the intracellular glutathione.
CME also increased tail moment value and H2AX phosphorylation in HCT 116 cells, suggesting DNA damage as an early signal of CME induced apoptosis.
Loss of mitochondrial membrane potential in CME-treated cells also indicated the involvement of mitochondria in CME induced apoptosis.
This study indicated the selectivity of CME toward colon cancer cells with the involvement of oxidative damage as its possible mechanism of action.
American Psychological Association (APA)
Leong, Lek Mun& Chan, Kok Meng& Hamid, Asmah& Latip, Jalifah& Rajab, Nor Fadilah. 2016. Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103967
Modern Language Association (MLA)
Leong, Lek Mun…[et al.]. Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1103967
American Medical Association (AMA)
Leong, Lek Mun& Chan, Kok Meng& Hamid, Asmah& Latip, Jalifah& Rajab, Nor Fadilah. Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103967