A Green Antioxidant Activity-Integrated Dual-Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On-Line DPPH-CE-DAD
Joint Authors
Chang, Yanxu
Gao, Xiu-Mei
Li, Jin
Liu, Jiao
Liu, Wei
Yu, Xie-an
Cao, Jun
An, Mingrui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Much attention has been focused on treatment of sepsis which leads to high mortality all over the world in every year.
Antioxidant activity seems to play a prominent role in the treatment of sepsis exhibited by Xuebijing injection.
The aim of the present research was to develop an on-line 1, 1-diphenyl-2-picrylhydrazyl- (DPPH-) capillary electrophoresis-diode array detector (on-line DPPH-CE-DAD) method for rapidly assessing antioxidant properties and efficacious material basis of antioxidant activity as a way of quality control of Xuebijing injection.
Several parameters affecting the separation were investigated, including the pH and concentrations of buffer, SDS, β-CD, and organic modifier as well as voltage and cassette temperature.
Compared to previous traditional method, this improved method shortened the experimental cycle and became more efficient because it was successfully applied to analyze total antioxidant activity and contents of twelve antioxidants of Xuebijing injection under the same condition.
The results revealed that the on-line DPPH-CE-DAD method was a reagent-saving, rapid, feasible, and green technique for quality control of Xuebijing injection in terms of pharmacological activity and contents of active ingredients.
It also offered new opportunities for the analysis of antioxidant activity of complex matrix.
American Psychological Association (APA)
Li, Jin& Liu, Jiao& Liu, Wei& Yu, Xie-an& Cao, Jun& An, Mingrui…[et al.]. 2016. A Green Antioxidant Activity-Integrated Dual-Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On-Line DPPH-CE-DAD. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104013
Modern Language Association (MLA)
Li, Jin…[et al.]. A Green Antioxidant Activity-Integrated Dual-Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On-Line DPPH-CE-DAD. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104013
American Medical Association (AMA)
Li, Jin& Liu, Jiao& Liu, Wei& Yu, Xie-an& Cao, Jun& An, Mingrui…[et al.]. A Green Antioxidant Activity-Integrated Dual-Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On-Line DPPH-CE-DAD. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104013