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Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3KAKT Pathway
Joint Authors
Sun, Jia-Qi
Zhang, Yi
Nan, Nan
Sun, Xu
Yu, Ming-Wei
Wang, Hong
Wang, Xiao-Min
Li, Jin-Ping
Zhang, Gan-lin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Although Chinese herbal compounds have long been alternatively applied for cancer treatment in China, their treatment effects have not been sufficiently investigated.
The Chinese herb Spatholobus suberectus is commonly prescribed to cancer patients.
HPLC analysis has shown that the main components of Spatholobus suberectus are flavonoids that can be classified as phytoestrogens, having a structure similar to estrogen.
This study was designed to investigate the effects of Spatholobus suberectus column extract (SSCE) on the estrogen receptor-positive (ER+) breast cancer cell line MCF-7 and its possible molecular mechanism.
In our study, MTT assay was performed to evaluate cell viability.
The results show that SSCE (80, 160, and 320 μg/ml) significantly decreased the viability of MCF-7 cells.
SSCE also triggered apoptosis, arrested the cell cycle at the G0/G1 phase, and inhibited cell migration.
A dual-luciferase reporter system showed that SSCE suppressed intranuclear p-ER activity; Western blot analysis confirmed the repressed expression of phosphorylated-ER alpha (p-ERα), ERK1/2, p-ERK1/2, AKT, p-AKT, p-mTOR, PI3K, and p-PI3K, indicating that SSCE suppressed the MAPK PI3K/AKT signaling pathway.
Collectively, our results suggest that SSCE causes apoptosis, an arrest in the G0/G1 phase, and a decrease in migration in ER+ MCF-7 cells via hypoactivity of the ER and suppression of the MAPK PI3K/AKT pathway.
American Psychological Association (APA)
Sun, Jia-Qi& Zhang, Gan-lin& Zhang, Yi& Nan, Nan& Sun, Xu& Yu, Ming-Wei…[et al.]. 2016. Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104030
Modern Language Association (MLA)
Sun, Jia-Qi…[et al.]. Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104030
American Medical Association (AMA)
Sun, Jia-Qi& Zhang, Gan-lin& Zhang, Yi& Nan, Nan& Sun, Xu& Yu, Ming-Wei…[et al.]. Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3KAKT Pathway. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104030
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104030