![](/images/graphics-bg.png)
Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen-Induced Arthritis in Rats
Joint Authors
Xie, Wen-xia
Huang, Ka-te
Du, Zhong-heng
Ye, Tian-shen
Wang, Junlu
Li, Zhi-hui
Chen, Yong
Chen, Zhou-yang
Hu, Huan
Fang, Jian-Qiao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this paper was to investigate the effect of repeated electroacupuncture (EA) over 21 days on the adenosine concentration in peripheral blood of rats with collagen-induced arthritis (CIA).
Wistar rats were divided into three groups of 6 animals each: sham-control, CIA-control, and CIA-EA.
We determined the adenosine concentration in peripheral blood and assessed pathological changes of ankle joints.
Quantitative reverse-transcription-polymerase chain reaction was used to determine mRNA levels of ecto-5′-nucleotidase (CD73), adenosine deaminase (ADA), and tumor necrosis factor-alpha (TNF-α).
Immunohistochemical staining was used to detect expression of ADA and CD73 in synovial tissue.
Repeated EA treatment on CIA resulted in the persistence of high concentrations of adenosine in peripheral blood, significantly reduced pathological scores, TNF-α mRNA concentrations, and synovial hyperplasia.
Importantly, EA treatment led to a significant increase in CD73 mRNA levels in peripheral blood but was associated with a decrease of CD73 immunostaining in synovial tissue.
In addition, EA treatment resulted in a significant decrease of both ADA mRNA levels in peripheral blood and ADA immunostaining in synovial tissue.
Thus, repeated EA treatment exerts an anti-inflammatory and immunoregulatory effect on CIA by increasing the concentration of adenosine.
The mechanism of EA action may involve the modulation of CD73 and ADA expression levels.
American Psychological Association (APA)
Ye, Tian-shen& Du, Zhong-heng& Li, Zhi-hui& Xie, Wen-xia& Huang, Ka-te& Chen, Yong…[et al.]. 2016. Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104090
Modern Language Association (MLA)
Ye, Tian-shen…[et al.]. Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1104090
American Medical Association (AMA)
Ye, Tian-shen& Du, Zhong-heng& Li, Zhi-hui& Xie, Wen-xia& Huang, Ka-te& Chen, Yong…[et al.]. Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104090