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Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera
Joint Authors
Berdja, Sihem
Smail, Leila
Saka, Boualem
Neggazi, Samia
Haffaf, El-mehdi
Benazzoug, Yasmina
Kacimi, Ghouti
Boudarene, Lynda
Aouichat Bouguerra, Souhila
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Context.
Brassica rapa is considered as natural source of antioxidants and is used to treat diabetes.
Objective.
Our study carried the impact of glucotoxicity induced in vivo and in vitro in vascular smooth muscle cells (VSMCs) in Psammomys and the therapeutic effect of Brassica rapa (AEBr).
Materials and Methods.
We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100 mg/kg.
VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100 μg/mL) for 24 hours.
We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue.
Histological study was effectuated in liver.
In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-κB) and oxidative stress (oxidants and antioxydants markers).
Cell viability and apoptosis were estimated by the morphological study.
Results.
AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver.
It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-κB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity.
Conclusion.
Brassica enhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases.
American Psychological Association (APA)
Berdja, Sihem& Smail, Leila& Saka, Boualem& Neggazi, Samia& Haffaf, El-mehdi& Benazzoug, Yasmina…[et al.]. 2016. Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1104094
Modern Language Association (MLA)
Berdja, Sihem…[et al.]. Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1104094
American Medical Association (AMA)
Berdja, Sihem& Smail, Leila& Saka, Boualem& Neggazi, Samia& Haffaf, El-mehdi& Benazzoug, Yasmina…[et al.]. Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1104094
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104094