Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway
Joint Authors
Im, Eun Ju
Kim, Su Jung
Hong, Seung Bok
Park, Jin-Kyu
Rhee, Man Hee
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Bee venom has long been used as a traditional folk medicine in Korea.
It has been reportedly used for the treatment of arthritis, cancer, and inflammation.
Although its anti-inflammatory activity in lipopolysaccharide- (LPS-) stimulated inflammatory cells has been reported, the exact mechanism of its anti-inflammatory action has not been fully elucidated.
Therefore, the aim of this study was to investigate the anti-inflammatory mechanism of bee venom in BV2 microglial cells.
We first investigated whether NO production in LPS-activated BV2 cells was inhibited by bee venom, and further iNOS mRNA and protein expressions were determined.
The mRNA and protein levels of proinflammatory cytokines were examined using semiquantitative RT-PCR and immunoblotting, respectively.
Moreover, modulation of the transcription factor NF-κB by bee venom was also investigated using a luciferase assay.
LPS-induced NO production in BV2 microglial cells was significantly inhibited in a concentration-dependent manner upon pretreatment with bee venom.
Bee venom markedly reduced the mRNA expression of COX-2, TNF-α, IL-1β, and IL-6 and suppressed LPS-induced activation of MyD88 and IRAK1 and phosphorylation of TAK1.
Moreover, NF-κB translocation by IKKα/β phosphorylation and subsequent IκB-α degradation were also attenuated.
Thus, collectively, these results indicate that bee venom exerts its anti-inflammatory activity via the IRAK1/TAK1/NF-κB signaling pathway.
American Psychological Association (APA)
Im, Eun Ju& Kim, Su Jung& Hong, Seung Bok& Park, Jin-Kyu& Rhee, Man Hee. 2016. Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104097
Modern Language Association (MLA)
Im, Eun Ju…[et al.]. Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104097
American Medical Association (AMA)
Im, Eun Ju& Kim, Su Jung& Hong, Seung Bok& Park, Jin-Kyu& Rhee, Man Hee. Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104097
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104097