Icariin Attenuates OGDR-Induced Autophagy via Bcl-2-Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells
Joint Authors
Mo, Zhen-tao
Li, Wen-na
Zhai, Yu-rong
Gong, Qi-hai
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Icariin (ICA), an active component of Epimedium brevicornum Maxim, exerts a variety of neuroprotective effects such as antiapoptosis.
However, the mechanisms underlying antiapoptosis of ICA in neurons exposed to oxygen-glucose deprivation and reperfusion (OGD/R) are unclear.
The B-cell lymphoma-2 (Bcl-2) protein family plays an important role in the regulation of apoptosis and autophagy through Bcl-2-dependent cross talk.
Bcl-2 suppresses apoptosis by binding to Bax and inhibits autophagy by binding to Beclin-1 which is an autophagy related protein.
In the present study, MTT result showed that ICA increased cell viability significantly in OGD/R treated PC12 cells ( P < 0.01 ).
Results of western blotting analysis showed that ICA increased Bcl-2 expression significantly and decreased expressions of Bax, cleaved Caspase-3, Beclin-1, and LC3-II significantly in OGD/R treated PC12 cells ( P < 0.01 ).
These results suggest that ICA protects PC12 cells from OGD/R induced autophagy via Bcl-2-dependent cross talk between apoptosis and autophagy.
American Psychological Association (APA)
Mo, Zhen-tao& Li, Wen-na& Zhai, Yu-rong& Gong, Qi-hai. 2016. Icariin Attenuates OGDR-Induced Autophagy via Bcl-2-Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104142
Modern Language Association (MLA)
Mo, Zhen-tao…[et al.]. Icariin Attenuates OGDR-Induced Autophagy via Bcl-2-Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104142
American Medical Association (AMA)
Mo, Zhen-tao& Li, Wen-na& Zhai, Yu-rong& Gong, Qi-hai. Icariin Attenuates OGDR-Induced Autophagy via Bcl-2-Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104142