Effects of Yiqi Tongyang on HCN4 Protein Phosphorylation in Damaged Rabbit Sinoatrial Node Cells
Joint Authors
Liu, Jinfeng
Liu, Ruxiu
Peng, Jie
Wang, Yanli
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The hyperpolarization-activated cyclic nucleotide-gated cation channel (If) is closely associated with sinoatrial node pacing function.
The present study aimed to investigate the molecular mechanisms involved in pacing function improvements of damaged sinoatrial node cells and the consequent treatment effects on sick sinus syndrome (SSS) after the use of Yiqi Tongyang.
HCN4 channel protein expression and phosphorylation were measured by immunoblotting and fluorescent quantitation.
After ischemia-reperfusion injury (model group), the HCN4 protein and the optical density (OD) of the phosphorylated HCN4 protein as well as intracellular PKA activity in the sinoatrial node cells decreased significantly.
However, the OD values and PKA activity increased to different degrees after treatment with serum containing different doses of Yiqi Tongyang; in contrast, no significant improvement was seen in the control group compared to the model group.
These findings demonstrated that the use of the traditional Chinese medicine Yiqi Tongyang could increase HCN4 protein expression and phosphorylation as well as PKA activity within sinoatrial node cells damaged by ischemia-reperfusion.
The HCN4 protein is involved in the If-related ion channel.
Here, we speculated that these effects could be associated with upregulation of HCN4 protein phosphorylation, which consequently improved cell automaticity, increased heart rate, and had treatment effects on SSS.
American Psychological Association (APA)
Liu, Jinfeng& Liu, Ruxiu& Peng, Jie& Wang, Yanli. 2016. Effects of Yiqi Tongyang on HCN4 Protein Phosphorylation in Damaged Rabbit Sinoatrial Node Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104148
Modern Language Association (MLA)
Liu, Jinfeng…[et al.]. Effects of Yiqi Tongyang on HCN4 Protein Phosphorylation in Damaged Rabbit Sinoatrial Node Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104148
American Medical Association (AMA)
Liu, Jinfeng& Liu, Ruxiu& Peng, Jie& Wang, Yanli. Effects of Yiqi Tongyang on HCN4 Protein Phosphorylation in Damaged Rabbit Sinoatrial Node Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104148