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Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from Philippine Piper betle L. against Multidrug-Resistant Bacteria
Joint Authors
Rivera, Windell L.
Cabrera, Esperanza C.
Valle, Demetrio L.
Puzon, Juliana Janet M.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study isolated and identified the antimicrobial compounds of Philippine Piper betle L.
leaf ethanol extracts by thin layer chromatography- (TLC-) bioautography and gas chromatography-mass spectrometry (GC-MS).
Initially, TLC separation of the leaf ethanol extracts provided a maximum of eight compounds with R f values of 0.92, 0.86, 0.76, 0.53, 0.40, 0.25, 0.13, and 0.013, best visualized when inspected under UV 366 nm.
Agar-overlay bioautography of the isolated compounds demonstrated two spots with R f values of 0.86 and 0.13 showing inhibitory activities against two Gram-positive multidrug-resistant (MDR) bacteria, namely, methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus.
The compound with an R f value of 0.86 also possessed inhibitory activity against Gram-negative MDR bacteria, namely, carbapenem-resistant Enterobacteriaceae-Klebsiella pneumoniae and metallo-β-lactamase-producing Acinetobacter baumannii.
GC-MS was performed to identify the semivolatile and volatile compounds present in the leaf ethanol extracts.
Six compounds were identified, four of which are new compounds that have not been mentioned in the medical literature.
The chemical compounds isolated include ethyl diazoacetate, tris(trifluoromethyl)phosphine, heptafluorobutyrate, 3-fluoro-2-propynenitrite, 4-(2-propenyl)phenol, and eugenol.
The results of this study could lead to the development of novel therapeutic agents capable of dealing with specific diseases that either have weakened reaction or are currently not responsive to existing drugs.
American Psychological Association (APA)
Valle, Demetrio L.& Puzon, Juliana Janet M.& Cabrera, Esperanza C.& Rivera, Windell L.. 2016. Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from Philippine Piper betle L. against Multidrug-Resistant Bacteria. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104179
Modern Language Association (MLA)
Valle, Demetrio L.…[et al.]. Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from Philippine Piper betle L. against Multidrug-Resistant Bacteria. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104179
American Medical Association (AMA)
Valle, Demetrio L.& Puzon, Juliana Janet M.& Cabrera, Esperanza C.& Rivera, Windell L.. Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from Philippine Piper betle L. against Multidrug-Resistant Bacteria. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104179