Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a
Joint Authors
Wu, Wanyin
Zheng, Fang
Wu, Jingjing
Li, Xiong
Tang, Qing
Yang, LiJun
Yang, Xiaobing
Hann, Swei Sunny
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-04
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The aim of this study is to investigate the actions of Chinese herbal medicine, called “Fuzheng Kang-Ai” (FZKA for short) decoction, against non-small cell lung cancer (NSCLC) and its mechanisms in vitro and in vivo.
We showed that the effect of FZKA decoction significantly inhibited growth of A549 and PC9 cells.
Furthermore, FZKA increased phosphorylation of AMP-activated protein kinase alpha (AMPKα) and induced protein expression of insulin-like growth factor (IGF) binding protein 1 (IGFBP1) and forkhead homeobox type O3a (FOXO3a).
The specific inhibitor of AMPKα (Compound C) blocked FZKA-induced protein expression of IGFBP1 and FOXO3a.
Interestingly, silencing of IGFBP1 and FOXO3a overcame the inhibitory effect of FZKA on cell growth.
Moreover, silencing of IGFBP1 attenuated the effect of FZKA decoction on FOXO3a expression, and exogenous expression of FOXO3a enhanced the FZKA-stimulated phosphorylation of AMPKα.
Accordingly, FZKA inhibited the tumor growth in xenograft nude mice model.
Collectively, our results show that FZKA decoction inhibits proliferation of NSCLC cells through activation of AMPKα, followed by induction of IGFBP1 and FOXO3a proteins.
Exogenous expression of FOXO3a feedback enhances FZKA decoction-stimulated IGFBP1 expression and phosphorylation of AMPKα.
The reciprocal interplay of IGFBP1 and FOXO3a contribute to the overall responses of FAKA decoction.
American Psychological Association (APA)
Zheng, Fang& Wu, Jingjing& Li, Xiong& Tang, Qing& Yang, LiJun& Yang, Xiaobing…[et al.]. 2016. Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104187
Modern Language Association (MLA)
Zheng, Fang…[et al.]. Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1104187
American Medical Association (AMA)
Zheng, Fang& Wu, Jingjing& Li, Xiong& Tang, Qing& Yang, LiJun& Yang, Xiaobing…[et al.]. Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104187