Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment
Joint Authors
Souza, Celeste da Silva Freitas
Almeida-Souza, Fernando
Taniwaki, Noemi Nosomi
Amaral, Ana Cláudia Fernandes
Abreu-Silva, Ana Lúcia
Calabrese, Kátia da Silva
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV.
Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature.
Studies have shown the leishmanicidal activity of M.
citrifolia; however, its action on the parasite has not yet been elucidated.
In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M.
citrifolia fruit juice treatment.
M.
citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents.
IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L.
infantum treated with M.
citrifolia.
Cytotoxicity assay with J774.G8 macrophages showed that M.
citrifolia fruit juice was not toxic up to 2 mg/mL.
Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L.
infantum promastigotes treated with M.
citrifolia fruit juice.
M.
citrifolia fruit juice was active against L.
infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis.
American Psychological Association (APA)
Almeida-Souza, Fernando& Taniwaki, Noemi Nosomi& Amaral, Ana Cláudia Fernandes& Souza, Celeste da Silva Freitas& Calabrese, Kátia da Silva& Abreu-Silva, Ana Lúcia. 2016. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104188
Modern Language Association (MLA)
Almeida-Souza, Fernando…[et al.]. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1104188
American Medical Association (AMA)
Almeida-Souza, Fernando& Taniwaki, Noemi Nosomi& Amaral, Ana Cláudia Fernandes& Souza, Celeste da Silva Freitas& Calabrese, Kátia da Silva& Abreu-Silva, Ana Lúcia. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104188