Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome
Joint Authors
Liu, Changming
Mao, Liangen
Ping, Zepeng
Jiang, Tingting
Wang, Chong
Chen, Zhongliang
Li, Zhongjie
Li, Jicheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Yin-deficiency-heat (YDH) syndrome is a concept in Traditional Chinese Medicine (TCM) for describing subhealth status.
However, there are few efficient diagnostic methods available for confirming YDH syndrome.
To explore the novel method for diagnosing YDH syndrome, we applied iTRAQ to observe the serum protein profiles in YDH syndrome rats and confirmed protein levels by ELISA.
A total of 92 differentially expressed proteins (63 upregulated proteins and 29 downregulated proteins), which were mainly involved in complement and coagulation cascades and glucose metabolism pathway, were identified by the proteomic experiments.
Kininogen 1 (KNG1) was significantly increased ( p < 0.0001 ), while apolipoprotein C-III (APOC3, p < 0.005 ) and paraoxonase 1 (PON1, p < 0.001 ) were significantly decreased in the serum of YDH syndrome rats.
The combination of KNG1, APOC3, and PON1 constituted a diagnostic model with 100.0% sensitivity and 85.0% specificity.
The results indicated that KNG1, APOC3, and PON1 may act as potential biomarkers for diagnosing YDH syndrome.
KNG1 may regulate cytokines and chemokines release in YDH syndrome, and the low levels of PON1 and APOC3 may increase oxidative stress and lipolysis in YDH syndrome, respectively.
Our work provides a novel method for YDH syndrome diagnosis and also provides valuable experimental basis to understand the molecular mechanism of YDH syndrome.
American Psychological Association (APA)
Liu, Changming& Mao, Liangen& Ping, Zepeng& Jiang, Tingting& Wang, Chong& Chen, Zhongliang…[et al.]. 2016. Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104196
Modern Language Association (MLA)
Liu, Changming…[et al.]. Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104196
American Medical Association (AMA)
Liu, Changming& Mao, Liangen& Ping, Zepeng& Jiang, Tingting& Wang, Chong& Chen, Zhongliang…[et al.]. Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104196
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104196