Selective Inhibition of Bakuchicin Isolated from Psoralea corylifolia on CYP1A in Human Liver Microsomes
Joint Authors
Kim, Sun Joo
Oh, Heung Chan
Kim, Youn-Chul
Jeong, Gil-Saeng
Lee, Sangkyu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bakuchicin is a furanocoumarin isolated from Psoralea corylifolia and shows several biological activities.
Although there have been studies on the biological effects of bakuchicin, its modulation potency of CYP activities has not been previously investigated.
Here, we investigated the inhibitory effects of bakuchicin on the activities of CYP isoforms by using a cocktail of probe substrates in pooled human liver microsomes (HLMs) and human recombinant cDNA-expressed CYP.
Bakuchicin strongly inhibited CYP1A-mediated phenacetin O-deethylation with an IC50 value of 0.43 μM in HLMs.
It was confirmed by human recombinant cDNA-expressed CYP1A1 and CYP1A2 with a Ki value of 0.11 μM and 0.32 μM, respectively.
A Lineweaver-Burk plot indicated that the inhibition mechanism of bakuchicin was competitive inhibition.
Overall, this is the first study to investigate the potential CYP1A1 and CYP1A2 inhibition associated with bakuchicin and to report its competitive inhibitory effects on HLMs.
American Psychological Association (APA)
Kim, Sun Joo& Oh, Heung Chan& Kim, Youn-Chul& Jeong, Gil-Saeng& Lee, Sangkyu. 2016. Selective Inhibition of Bakuchicin Isolated from Psoralea corylifolia on CYP1A in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104197
Modern Language Association (MLA)
Kim, Sun Joo…[et al.]. Selective Inhibition of Bakuchicin Isolated from Psoralea corylifolia on CYP1A in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104197
American Medical Association (AMA)
Kim, Sun Joo& Oh, Heung Chan& Kim, Youn-Chul& Jeong, Gil-Saeng& Lee, Sangkyu. Selective Inhibition of Bakuchicin Isolated from Psoralea corylifolia on CYP1A in Human Liver Microsomes. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104197
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104197