Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts
Joint Authors
Gustafsson, A.
Ocheng, Francis
Bwanga, Freddie
Borg-Karlson, Anna-Karin
Obua, Celestino
Almer Boström, Elisabeth
Yucel-Lindberg, Tülay
Joloba, Moses L.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The study investigated cytotoxicity of essential oils from four medicinal plants (Bidens pilosa, Ocimum gratissimum, Cymbopogon nardus, and Zanthoxylum chalybeum) on human gingival fibroblasts and their effects on proinflammatory mediators’ secretion.
Cytotoxicity of essential oils was investigated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay.
Effects of essential oils at subcytotoxicity concentrations on interleukin- (IL-) 6, IL-8, and prostaglandin E2 (PGE2) secretions by gingival fibroblasts treated with IL-1β (300 pg/mL) were evaluated by ELISA and EIA.
IC50 values of the essential oils ranged from 26 μg/mL to 50 μg/mL.
Baseline and IL-1β-induced secretion of PGE2 was inhibited by treatment with essential oil from O.
gratissimum.
Essential oils from B.
pilosa and C.
nardus had synergistic effects with IL-1β on PGE2 seceretion.
In conclusion, the study suggests that essential oil from O.
gratissimum decreases gingival fibroblasts secretion of PGE2, while essential oils from B.
pilosa and C.
nardus increase PGE2 secretion.
Essential oil from Z.
chalybeum was the most cytotoxic, while oil from C.
nardus was the least cytotoxic.
Although the clinical significance of these findings remains to be determined, it may be suggested that essential oil from O.
gratissimum, applied at subcytotoxicity concentrations, could reduce the participation of gingival fibroblasts in the gingival inflammation and tissue destruction associated with periodontitis.
American Psychological Association (APA)
Ocheng, Francis& Bwanga, Freddie& Almer Boström, Elisabeth& Joloba, Moses L.& Borg-Karlson, Anna-Karin& Yucel-Lindberg, Tülay…[et al.]. 2016. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104205
Modern Language Association (MLA)
Ocheng, Francis…[et al.]. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1104205
American Medical Association (AMA)
Ocheng, Francis& Bwanga, Freddie& Almer Boström, Elisabeth& Joloba, Moses L.& Borg-Karlson, Anna-Karin& Yucel-Lindberg, Tülay…[et al.]. Essential Oils from Ugandan Medicinal Plants: In Vitro Cytotoxicity and Effects on IL-1β-Induced Proinflammatory Mediators by Human Gingival Fibroblasts. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104205