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Effect of Aurantii Fructus Immaturus Flavonoid on the Contraction of Isolated Gastric Smooth Muscle Strips in Rats
Joint Authors
Zhao, Luqing
Zhang, Shengsheng
Wu, Zhenyu
Lu, Xiaofang
Li, Peicai
Wang, Jiajia
Wang, Yueqi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study was designed to investigate the effect of Aurantii fructus immaturus flavonoid (AFIF) on the contraction of isolated gastric smooth muscle in rats and explore its underlying mechanisms.
Isolated antral longitudinal smooth muscle strip (ALSMS) and pyloric circular smooth muscle strip (PCSMS) of rats were suspended in tissue chambers.
The responses of ALSMS and PCSMS to administration of AFIF were observed.
Cyclic guanosine monophosphate (cGMP) and protein kinase G (PKG) levels of PCSMS were measured by ELISA kits.
In this study, AFIF showed no significant effect on ALSMS contraction, but it dose-dependently reduced the mean contraction amplitude of PCSMS.
When the concentration of AFIF reached 3000 μ g/mL, 6000 μ g/mL, and 10000 μ g/mL, its inhibitory effect on PCSMS contraction was significant.
This effect of AFIF was weakened in Ca2+-rich environment.
And N ω -nitro-L-arginine methyl (L-NAME), the inhibitor of nitric oxide synthase (NOS), significantly inhibited AFIF’s action in comparison with control ( P < 0.05 ).
After incubation with AFIF for 30 min, levels of cGMP and PKG in PCSMS were significantly increased compared with control ( P < 0.05 ).
Our results suggest that AFIF has a dose-dependent diastolic effect on PCSMS in rats, which may be related to the regulatory pathway of NO/cGMP/PKG/Ca2+.
American Psychological Association (APA)
Wu, Zhenyu& Zhang, Shengsheng& Li, Peicai& Lu, Xiaofang& Wang, Jiajia& Zhao, Luqing…[et al.]. 2016. Effect of Aurantii Fructus Immaturus Flavonoid on the Contraction of Isolated Gastric Smooth Muscle Strips in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104217
Modern Language Association (MLA)
Wu, Zhenyu…[et al.]. Effect of Aurantii Fructus Immaturus Flavonoid on the Contraction of Isolated Gastric Smooth Muscle Strips in Rats. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104217
American Medical Association (AMA)
Wu, Zhenyu& Zhang, Shengsheng& Li, Peicai& Lu, Xiaofang& Wang, Jiajia& Zhao, Luqing…[et al.]. Effect of Aurantii Fructus Immaturus Flavonoid on the Contraction of Isolated Gastric Smooth Muscle Strips in Rats. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104217
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104217