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Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis
Joint Authors
Alviano, Daniela S.
Alviano, Celuta S.
Rodrigues, Igor A.
Blank, Arie Fitzgerald
Cardoso, Nathalia N. R.
Romanos, Maria Teresa V.
Fonseca, Beatriz B.
Rozental, Sonia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to evaluate the activity of the EO and its major components of Ocimum basilicum var.
Maria Bonita, a genetically improved cultivar, against the fluconazole sensitive and resistant strains of Candida albicans and Cryptococcus neoformans.
Geraniol presented better results than the EO, with a low MIC (76 μg/mL against C.
neoformans and 152 μg/mL against both Candida strains).
The combination of EO, linalool, or geraniol with fluconazole enhanced their antifungal activity, especially against the resistant strain (MIC reduced to 156, 197, and 38 μg/mL, resp.).
The ergosterol assay showed that subinhibitory concentrations of the substances were able to reduce the amount of sterol extracted.
The substances tested were able to reduce the capsule size which suggests they have an important mechanism of action.
Transmission electron microscopy demonstrated cell wall destruction of C.
neoformans after treatment with subinhibitory concentrations.
In C.
albicans ultrastructure alterations such as irregularities in the membrane, presence of vesicles, and cell wall thickening were observed.
The biofilm formation was inhibited in both C.
albicans strains at MIC and twice MIC.
These results provide further support for the use of O.
basilicum EO and its major components as a potential source of antifungal agents.
American Psychological Association (APA)
Cardoso, Nathalia N. R.& Alviano, Celuta S.& Blank, Arie Fitzgerald& Romanos, Maria Teresa V.& Fonseca, Beatriz B.& Rozental, Sonia…[et al.]. 2016. Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104219
Modern Language Association (MLA)
Cardoso, Nathalia N. R.…[et al.]. Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1104219
American Medical Association (AMA)
Cardoso, Nathalia N. R.& Alviano, Celuta S.& Blank, Arie Fitzgerald& Romanos, Maria Teresa V.& Fonseca, Beatriz B.& Rozental, Sonia…[et al.]. Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104219