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Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis
Joint Authors
Hu, Yonghong
Liu, Yafei
Chen, Zhe
Wen, Cai-Yu-Zhu
Huang, Ying
Tu, Shenghao
Wang, Yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Wutou decoction (WTD) has been wildly applied in the treatment of rheumatoid arthritis and experimental arthritis in rats for many years.
Epigenetic deregulation is associated with the aetiology of rheumatoid arthritis; however, the effects of WTD on epigenetic changes are unclear.
This study is set to explore the effects of WTD on DNA methylation and histone modifications in rats with collagen-induced arthritis (CIA).
Methods.
The CIA model was established by the stimulation of collagen and adjuvant.
The knee synovium was stained with hematoxylin and eosin.
The DNA methyltransferase 1 (DNMT1) and methylated CpG binding domain 2 (MBD2) expression of peripheral blood mononuclear cells (PBMCs) were determined by Real-Time PCR.
The global DNA histone H3-K4/H3-K27 methylation and total histones H3 and H4 acetylation of PBMCs were detected.
Results.
Our data demonstrated that the DNMT1 mRNA expression was significantly lowered in group WTD compared to that in group CIA ( P < 0.05 ).
The DNA methylation level was significantly reduced in group WTD compared to that in group CIA ( P < 0.05 ).
Moreover, H3 acetylation of PBMCs was overexpressed in WTD compared with CIA ( P < 0.05 ).
Conclusions.
WTD may modulate DNA methylation and histone modifications, functioning as anti-inflammatory potential.
American Psychological Association (APA)
Liu, Yafei& Wen, Cai-Yu-Zhu& Chen, Zhe& Wang, Yu& Huang, Ying& Hu, Yonghong…[et al.]. 2016. Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104232
Modern Language Association (MLA)
Liu, Yafei…[et al.]. Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1104232
American Medical Association (AMA)
Liu, Yafei& Wen, Cai-Yu-Zhu& Chen, Zhe& Wang, Yu& Huang, Ying& Hu, Yonghong…[et al.]. Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104232