Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells
Joint Authors
Batumalaie, Kalaivani
Ismail, Ikram S.
Qvist, Rajes
Mohd Yusof, Kamaruddin
Zaman Safi, Sher
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-13
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Purpose.
Type 2 diabetes consists of progressive hyperglycemia and insulin resistance, which could result from glucose toxicity, inflammatory cytokines, and oxidative stress.
In the present study we investigated the effect of Gelam honey and quercetin on the oxidative stress-induced inflammatory pathways and the proinflammatory cytokines.
Methods.
HIT-T15 cells were cultured and preincubated with the extract of Gelam honey (20, 40, 60, and 80 μg/mL), as well as quercetin (20, 40, 60, and 80 μM), prior to stimulation by 20 and 50 mM glucose.
Results.
HIT-T15 cells cultured under hyperglycemic condition showed a significant increase in the inflammatory pathways by phosphorylating JNK, IKK-β, and IRS-1 at Ser307 ( p < 0.05 ).
There was a significant decrease in the phosphorylation of Akt at Ser473 ( p < 0.05 ).
Pretreatment with Gelam honey and quercetin reduced the expression of phosphorylated JNK, IKK-β, and IRS-1, thereby significantly reducing the expression of proinflammatory cytokines like TNF-α, IL-6, and IL-1β ( p < 0.05 ).
At the same time there was a significant increase in the phosphorylated Akt showing the protective effects against inflammation and insulin resistance ( p < 0.05 ).
In conclusion, our data suggest the potential use of the extract from Gelam honey and quercetin in modulating the inflammation induced insulin signaling pathways.
American Psychological Association (APA)
Zaman Safi, Sher& Batumalaie, Kalaivani& Qvist, Rajes& Mohd Yusof, Kamaruddin& Ismail, Ikram S.. 2016. Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104233
Modern Language Association (MLA)
Zaman Safi, Sher…[et al.]. Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104233
American Medical Association (AMA)
Zaman Safi, Sher& Batumalaie, Kalaivani& Qvist, Rajes& Mohd Yusof, Kamaruddin& Ismail, Ikram S.. Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104233