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Supercritical Fluid Extract of Spent Coffee Grounds Attenuates Melanogenesis through Downregulation of the PKA, PI3KAkt, and MAPK Signaling Pathways
Joint Authors
Chang, Tsong-Min
Wei, Chien-Mei
Siao, Jen-Hung
Tsai, Tsang-Chi
Ko, Wang-Ping
Chang, Kuei-Jen
Hii, Choon-Hoon
Huang, Huey-Chun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The mode of action of spent coffee grounds supercritical fluid CO2 extract (SFE) in melanogenesis has never been reported.
In the study, the spent coffee grounds were extracted by the supercritical fluid CO2 extraction method; the chemical constituents of the SFE were investigated by gas chromatography-mass spectrometry (GC-MS).
The effects of the SFE and its major fatty acid components on melanogenesis were evaluated by mushroom tyrosinase activity assay and determination of intracellular tyrosinase activity and melanin content.
The expression level of melanogenesis-related proteins was analyzed by western blotting assay.
The results revealed that the SFE of spent coffee grounds (1–10 mg/mL) and its major fatty acids such as linoleic acid and oleic acid (6.25–50 μM) effectively suppressed melanogenesis in the B16F10 murine melanoma cells.
Furthermore, the SFE decreased the expression of melanocortin 1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2).
The SFE also decreased the protein expression levels of p-JNK, p-p38, p-ERK, and p-CREB.
Our results revealed that the SFE of spent coffee grounds attenuated melanogenesis in B16F10 cells by downregulation of protein kinase A (PKA), phosphatidylinositol-3-kinase (PI3K/Akt), and mitogen-activated protein kinases (MAPK) signaling pathways, which may be due to linoleic acid and oleic acid.
American Psychological Association (APA)
Huang, Huey-Chun& Wei, Chien-Mei& Siao, Jen-Hung& Tsai, Tsang-Chi& Ko, Wang-Ping& Chang, Kuei-Jen…[et al.]. 2016. Supercritical Fluid Extract of Spent Coffee Grounds Attenuates Melanogenesis through Downregulation of the PKA, PI3KAkt, and MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104236
Modern Language Association (MLA)
Huang, Huey-Chun…[et al.]. Supercritical Fluid Extract of Spent Coffee Grounds Attenuates Melanogenesis through Downregulation of the PKA, PI3KAkt, and MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1104236
American Medical Association (AMA)
Huang, Huey-Chun& Wei, Chien-Mei& Siao, Jen-Hung& Tsai, Tsang-Chi& Ko, Wang-Ping& Chang, Kuei-Jen…[et al.]. Supercritical Fluid Extract of Spent Coffee Grounds Attenuates Melanogenesis through Downregulation of the PKA, PI3KAkt, and MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104236
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104236