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Activation of p53miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells
Joint Authors
Yao, Chih-Jung
Bai, Kuan-Jen
Chow, Jyh-Ming
Kuo, Hui-Ching
Lin, Pei-Chun
Hu, Tsai-Shu
Huang, Jhy-Shrian
Lai, Gi-Ming
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Lung cancer is the leading cause of cancer death worldwide; the most common pathologic type is lung adenocarcinoma (LADC).
In spite of the recent progress in targeted therapy, most LADC patients eventually expired due to the inevitable recurrence and drug resistance.
New complementary agent with evidence-based molecular mechanism is urgently needed.
MiR-34a is an important p53 downstream tumor suppressor, which regulates apoptosis, cell-cycle, EMT (epithelial mesenchymal transition), and so forth.
Its expression is deficient in many types of cancers including LADC.
Here, we show that a Chinese herbal formula JP-1 activates p53/miR-34a axis in A549 human LADC cells (p53 wild-type).
Treatment with JP-1 induces p53 and its downstream p21 and BAX proteins as well as the miR-34a, resulting in growth inhibition, colony formation reduction, migration repression, and apoptosis induction.
Accordingly, the decreases of miR-34a downstream targets such as CDK6, SIRT1, c-Myc, survivin, Snail, and AXL were observed.
Moreover, JP-1 activates AMPKα and reduces mTOR activity, implying its inhibitory effect on the energy-sensitive protein synthesis and cell proliferation signaling.
Our results show that JP-1 activates p53/miR-34a tumor suppressor axis and decreases proteins related to proliferation, apoptosis resistance, and metastasis, suggesting its potential as a complementary medicine for LADC treatment.
American Psychological Association (APA)
Yao, Chih-Jung& Chow, Jyh-Ming& Lin, Pei-Chun& Hu, Tsai-Shu& Kuo, Hui-Ching& Huang, Jhy-Shrian…[et al.]. 2016. Activation of p53miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104252
Modern Language Association (MLA)
Yao, Chih-Jung…[et al.]. Activation of p53miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1104252
American Medical Association (AMA)
Yao, Chih-Jung& Chow, Jyh-Ming& Lin, Pei-Chun& Hu, Tsai-Shu& Kuo, Hui-Ching& Huang, Jhy-Shrian…[et al.]. Activation of p53miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1104252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104252