Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle
Joint Authors
Liu, Yali
Su, Dan
Zhang, Ling
Wei, Shaofeng
Liu, Kuangyi
Peng, Mi
Li, Hanyun
Song, Yonggui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A novel method for quantitation of cardiac muscle carnosine levels using HPLC-UV is described.
In this simple and reliable method, carnosine from the rat cardiac muscle and the internal standard, thymopentin, were extracted by protein precipitation with acetonitrile.
The method was linear up to 60.96 μg·mL−1 for L-carnosine.
The calibration curve was linear in concentration ranges from 0.5 to 60.96 μg·mL−1.
The relative standard deviations obtained for intra- and interday precision were lower than 12% and the recoveries were higher than 90% for both carnosine and internal standard.
We successfully applied this method to the analysis of endogenous carnosine in cardiac muscle of the diabetes rats and healthy control rats.
The concentration of carnosine was significantly lower in the diabetes rats group, compared to that in the healthy control rats.
These results support the usefulness of this method as a means of quantitating carnosine and illustrate the important role of L-carnosine in cardiac muscle.
American Psychological Association (APA)
Liu, Yali& Su, Dan& Zhang, Ling& Wei, Shaofeng& Liu, Kuangyi& Peng, Mi…[et al.]. 2016. Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104272
Modern Language Association (MLA)
Liu, Yali…[et al.]. Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104272
American Medical Association (AMA)
Liu, Yali& Su, Dan& Zhang, Ling& Wei, Shaofeng& Liu, Kuangyi& Peng, Mi…[et al.]. Endogenous L-Carnosine Level in Diabetes Rat Cardiac Muscle. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104272