Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells
Joint Authors
Sakaue, S.
Tamaki, Misako
Ogawa, Yui
Kaneki, Kiyomi
Zhang, Meng
Sunagawa, Masataka
Hisamitsu, T.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Osteoporosis is a serious public health problem characterized by low bone density and deterioration of the bone microarchitecture.
Current treatment options target either osteoclast resorption or osteoblast formation.
It has been reported that berberine, a close structural analog of palmatine, inhibited bone loss in an osteoporosis model.
In this study, osseous metabolism was observed in vitro with osteoclast bone resorbing cells.
We proved that mouse preosteoclastic cell line (RAW 264.7) has a higher sensitivity to palmatine than mouse osteoblastic cell line (MC3T3-E1); the cell survival rates significantly decreased at 40 μM palmatine.
The NO2- level, a metabolic product of nitric monoxide (NO), and iNOS mRNA expression, an osteoclast with NO induced enzyme, also increased with higher dosage of palmatine.
Furthermore, it was recognized that the cell viability decrease from palmatine was caused by apoptosis rather than necrosis.
Additionally, osteoclast apoptosis from palmatine did not occur when iNOS was inhibited with NG-nitro-L-arginine methyl ester hydrochloride (pan NOS inhibitor).
These results indicate that palmatine plays an important role in osteoclast apoptosis via the NOS system.
Hence, palmatine could be considered as a viable pharmaceutical candidate for osteoporosis bone resorption inhibitor.
American Psychological Association (APA)
Sakaue, S.& Tamaki, Misako& Ogawa, Yui& Kaneki, Kiyomi& Zhang, Meng& Sunagawa, Masataka…[et al.]. 2016. Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104332
Modern Language Association (MLA)
Sakaue, S.…[et al.]. Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1104332
American Medical Association (AMA)
Sakaue, S.& Tamaki, Misako& Ogawa, Yui& Kaneki, Kiyomi& Zhang, Meng& Sunagawa, Masataka…[et al.]. Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104332