Electroacupuncture Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulation of Autophagy and Apoptosis
Joint Authors
Ling, Chang-Quan
Shu, Shi
Li, Chun-Ming
You, Yan-Li
Qian, Xiao-Lu
Zhou, Shuang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
The therapeutic mechanisms of cerebral ischemia treatment by acupuncture are yet not well addressed.
Objective.
We investigated the effects of electroacupuncture (EA) at GV26 observing the expression of autophagy-related proteins Beclin-1 and LC3B and proportion of apoptotic cells and Bcl-2 positive cells in MCAO/R model rats.
Methods.
Sprague-Dawley (SD) male rats were randomly assigned to 7 groups: model groups (M6h, M24h, and M72h), EA treatment groups (T6h, T24h, and T72h), and sham operation group (S).
Neurological deficit and cerebral infarction volume were measured to assess the improvement effect, while the expression of Beclin-1 and LC3B and proportion of Tunel-positive and Bcl-2 positive cells were examined to explore EA effect on autophagy and apoptosis.
Results.
EA significantly decreased neurological deficit scores and the volume of cerebral infarction.
Beclin-1 was significantly decreased in T24h, while LC3B-II/LC3B-I ratio markedly reduced in 6th hour.
EA groups markedly reduced the number of Tunel positive cells, especially in T24h.
Meanwhile, the number of Bcl-2 positive cells obviously increased after EA treatment, especially in T6h and T24h.
Conclusions.
The alleviation of inadequate autophagy and apoptosis may be a key mechanism involved in the reflex regulation of EA at GV26 to treat cerebral ischemia.
American Psychological Association (APA)
Shu, Shi& Li, Chun-Ming& You, Yan-Li& Qian, Xiao-Lu& Zhou, Shuang& Ling, Chang-Quan. 2016. Electroacupuncture Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulation of Autophagy and Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104339
Modern Language Association (MLA)
Shu, Shi…[et al.]. Electroacupuncture Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulation of Autophagy and Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1104339
American Medical Association (AMA)
Shu, Shi& Li, Chun-Ming& You, Yan-Li& Qian, Xiao-Lu& Zhou, Shuang& Ling, Chang-Quan. Electroacupuncture Ameliorates Cerebral Ischemia-Reperfusion Injury by Regulation of Autophagy and Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104339
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104339