Effects of Extracts from Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I

Abstract EN

Sera from the rats with different drug treatments (atorvastatin, Tiaozhi granule, or its extracts) were collected.

LO-2 cells or HepG2 cells were pretreated with different sera as the following groups randomly: (1) blank control group, (2) positive control group (atorvastatin group), (3) Tiaozhi granule water extract groups, (4) Tiaozhi granule alcohol extract groups, and (5) alcohol extracts for each component: Pollen Typhae Angustifoliae, Curcuma longa L., and Rhizoma Alismatis.

LO-2 cells were cotransfected with plasmid carrying SR-BI and pRL-TK promoter genes.

Promoter activity was measured by the luciferase reporter gene assay.

The mRNA and protein expressions of SR-BI were examined using real-time PCR and western blot analyses.

Our results show that promoter activity and mRNA and protein expression levels of the SR-BI were significantly upregulated by Tiaozhi granules alcohol or water extracts in a dose-dependent manner.

Pollen Typhae Angustifoliae alcohol extract with a high dosage could also increase SR-BI activity and expression, but not the extracts from Curcuma longa L.

and Rhizoma Alismatis.

Both Tiaozhi granule alcohol and water extracts can upregulate SR-BI gene expression.

Among the components, Pollen Typhae Angustifoliae are important for the regulatory effect coordinating with Curcuma longa L.

and Rhizoma Alismatis.