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Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLCQ-TOF-MSMS)
Joint Authors
Shan, Lina
Yang, Shuman
Shi, Xianbao
Liu, Yali
Su, Dan
Song, Yonggui
Mackie, Brianna
Zhang, Gang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Morusin, the important active component of a traditional Chinese medicine, Morus alba L., has been shown to exhibit many vital pharmacological activities.
In this study, six recombinant CYP450 supersomes and liver microsomes were used to perform metabolic studies.
Chemical inhibition studies and screening assays with recombinant human cytochrome P450s were also used to characterize the CYP450 isoforms involved in morusin metabolism.
The morusin metabolites identified varied greatly among different species.
Eight metabolites of morusin were detected in the liver microsomes from pigs (PLMs), rats (RLMs), and monkeys (MLMs) by LC-MS/MS and six metabolites were detected in the liver microsomes from humans (HLMs), rabbits (RAMs), and dogs (DLMs).
Four metabolites (M1, M2, M5, and M7) were found in all species and hydroxylation was the major metabolic transformation.
CYP1A2, CYP2C9, CYP2D6, CYP2E1, CYP3A4, and CYP2C19 contributed differently to the metabolism of morusin.
Compared to other CYP450 isoforms, CYP3A4 played the most significant role in the metabolism of morusin in human liver microsomes.
These results are significant to better understand the metabolic behaviors of morusin among various species.
American Psychological Association (APA)
Shi, Xianbao& Mackie, Brianna& Zhang, Gang& Yang, Shuman& Song, Yonggui& Su, Dan…[et al.]. 2016. Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLCQ-TOF-MSMS). Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104484
Modern Language Association (MLA)
Shi, Xianbao…[et al.]. Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLCQ-TOF-MSMS). Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1104484
American Medical Association (AMA)
Shi, Xianbao& Mackie, Brianna& Zhang, Gang& Yang, Shuman& Song, Yonggui& Su, Dan…[et al.]. Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLCQ-TOF-MSMS). Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104484
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104484