Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3KAktmTOR Signaling Pathway
Joint Authors
Xu, Xiaolong
Wan, Changrong
Liu, Fenghua
Xu, Jianqin
Han, Dandan
Jiang, Linshu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Jujuboside A is a kind of the saponins isolated from the seeds of Ziziphus jujuba, which possesses multiple biological effects, such as antianxiety, antioxidant, and anti-inflammatory effects; however, its mediatory effect on isoproterenol-stimulated cardiomyocytes has not been investigated yet.
In this study, we tried to detect the protective effect and potential mechanism of JUA on ISO-induced cardiomyocytes injury.
H9C2 cells were treated with ISO to induce cell damage.
Cells were pretreated with JUA to investigate the effects on the cell viability, morphological changes, light chain 3 conversion, and the activation of PI3K/Akt/mTOR signaling pathway.
Results showed that ISO significantly inhibited the cell viability in a time- and dose-dependent manner.
JUA pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO.
Western blot analysis showed that JUA could accelerate the phosphorylation of PI3K, Akt, and mTOR.
Results also indicated that JUA could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells.
Taken together, our research showed that JUA could notably reduce the damage cause by ISO via promoting the phosphorylation of PI3K, Akt, and mTOR and inhibiting LC3 conversion, which may be a potential choice for the treatment of heart diseases.
American Psychological Association (APA)
Han, Dandan& Wan, Changrong& Liu, Fenghua& Xu, Xiaolong& Jiang, Linshu& Xu, Jianqin. 2016. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3KAktmTOR Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104517
Modern Language Association (MLA)
Han, Dandan…[et al.]. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3KAktmTOR Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1104517
American Medical Association (AMA)
Han, Dandan& Wan, Changrong& Liu, Fenghua& Xu, Xiaolong& Jiang, Linshu& Xu, Jianqin. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3KAktmTOR Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104517
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104517