miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease
Joint Authors
Zhang, Hui
Zhu, Shengliang
Yan, Xiuli
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-07
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background and Aim.
MicroRNAs (miRNAs) have been implicated in the pathophysiology of numerous human diseases including gastroesophageal reflux disease (GERD).
The objective of this study was to investigate the miRNA expression of exfoliated cells of the tongue in patients with GERD versus healthy controls (Ctrls).
Methods.
Using quantitative reverse-transcription PCR (qRT-PCR), expression levels of six candidate miRNAs (miR-143, miR-145, miR-192, miR-194, miR-203, and miR-205) were examined across a discovery cohort of patients with GERD ( n = 24 ) versus Ctrls ( n = 24 ).
These findings were confirmed across a validation cohort (GERD, n = 1 42 ; Ctrls, n = 48 ).
Differences in miRNA expression levels were evaluated using the Mann-Whitney U test while the specificity and sensitivity were obtained using receiver-operator characteristic (ROC) curves.
Results.
miR-203 was significantly downregulated in GERD patients as compared to Ctrls ( P < 0.0001 ) with ROC curve of 0.94 (95% CI: 0.90–0.97).
The sensitivity and the specificity of miR-203 were 91.7% and 87.3%, respectively, in the GERD and Ctrls.
These results suggest that miR-203 may be a useful diagnostic marker for discriminating GERD from Ctrls.
Conclusions.
miR-203 testing may assist in the diagnosis of patients with symptoms suggestive of GERD.
American Psychological Association (APA)
Yan, Xiuli& Zhu, Shengliang& Zhang, Hui. 2016. miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104614
Modern Language Association (MLA)
Yan, Xiuli…[et al.]. miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1104614
American Medical Association (AMA)
Yan, Xiuli& Zhu, Shengliang& Zhang, Hui. miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104614