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Genetic Abnormalities in Biliary Brush Samples for Distinguishing Cholangiocarcinoma from Benign Strictures in Primary Sclerosing Cholangitis
Joint Authors
Timmer, Margriet R.
Lau, Chiu T.
Fockens, Paul
Ponsioen, Cyriel Y.
Calpe, Silvia
Rauws, Erik A.
Krishnadath, Kausillia K.
Meijer, Sybren L.
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Primary sclerosing cholangitis (PSC) is a chronic inflammatory liver disease and is strongly associated with cholangiocarcinoma (CCA).
The lack of efficient diagnostic methods for CCA is a major problem.
Testing for genetic abnormalities may increase the diagnostic value of cytology.
Methods.
We assessed genetic abnormalities for CDKN2A, TP53, ERBB2, 20q, MYC, and chromosomes 7 and 17 and measures of genetic clonal diversity in brush samples from 29 PSC patients with benign biliary strictures and 12 patients with sporadic CCA or PSC-associated CCA.
Diagnostic performance of cytology alone and in combination with genetic markers was evaluated by sensitivity, specificity, and area under the curve analysis.
Results.
The presence of MYC gain and CDKN2A loss as well as a higher clonal diversity was significantly associated with malignancy.
MYC gain increased the sensitivity of cytology from 50% to 83%.
However, the specificity decreased from 97% to 76%.
The diagnostic accuracy of the best performing measures of clonal diversity was similar to the combination of cytology and MYC.
Adding CDKN2A loss to the panel had no additional benefit.
Conclusion.
Evaluation of MYC abnormalities and measures of clonal diversity in brush cytology specimens may be of clinical value in distinguishing CCA from benign biliary strictures in PSC.
American Psychological Association (APA)
Timmer, Margriet R.& Lau, Chiu T.& Meijer, Sybren L.& Fockens, Paul& Rauws, Erik A.& Ponsioen, Cyriel Y.…[et al.]. 2016. Genetic Abnormalities in Biliary Brush Samples for Distinguishing Cholangiocarcinoma from Benign Strictures in Primary Sclerosing Cholangitis. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104685
Modern Language Association (MLA)
Timmer, Margriet R.…[et al.]. Genetic Abnormalities in Biliary Brush Samples for Distinguishing Cholangiocarcinoma from Benign Strictures in Primary Sclerosing Cholangitis. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1104685
American Medical Association (AMA)
Timmer, Margriet R.& Lau, Chiu T.& Meijer, Sybren L.& Fockens, Paul& Rauws, Erik A.& Ponsioen, Cyriel Y.…[et al.]. Genetic Abnormalities in Biliary Brush Samples for Distinguishing Cholangiocarcinoma from Benign Strictures in Primary Sclerosing Cholangitis. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104685
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104685