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The Role of Chromosomal Instability and Epigenetics in Colorectal Cancers Lacking β-CateninTCF Regulated Transcription
Joint Authors
Abdel-Rahman, Wael M.
Lotsari-Salomaa, Johanna E.
Kaur, Sippy
Niskakoski, Anni
Järvinen, Heikki
Mecklin, Jukka-Pekka
Peltomäki, Päivi
Knuutila, Sakari
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
All colorectal cancer cell lines except RKO displayed active β-catenin/TCF regulated transcription.
This feature of RKO was noted in familial colon cancers; hence our aim was to dissect its carcinogenic mechanism.
MFISH and CGH revealed distinct instability of chromosome structure in RKO.
Gene expression microarray of RKO versus 7 colon cancer lines (with active Wnt signaling) and 3 normal specimens revealed 611 differentially expressed genes.
The majority of the tested gene loci were susceptible to LOH in primary tumors with various β-catenin localizations as a surrogate marker for β-catenin activation.
The immunohistochemistry of selected genes (IFI16, RGS4, MCTP1, DGKI, OBCAM/OPCML, and GLIPR1) confirmed that they were differentially expressed in clinical specimens.
Since epigenetic mechanisms can contribute to expression changes, selected target genes were evaluated for promoter methylation in patient specimens from sporadic and hereditary colorectal cancers.
CMTM3, DGKI, and OPCML were frequently hypermethylated in both groups, whereas KLK10, EPCAM, and DLC1 displayed subgroup specificity.
The overall fraction of hypermethylated genes was higher in tumors with membranous β-catenin.
We identified novel genes in colorectal carcinogenesis that might be useful in personalized tumor profiling.
Tumors with inactive Wnt signaling are a heterogeneous group displaying interaction of chromosomal instability, Wnt signaling, and epigenetics.
American Psychological Association (APA)
Abdel-Rahman, Wael M.& Lotsari-Salomaa, Johanna E.& Kaur, Sippy& Niskakoski, Anni& Knuutila, Sakari& Järvinen, Heikki…[et al.]. 2016. The Role of Chromosomal Instability and Epigenetics in Colorectal Cancers Lacking β-CateninTCF Regulated Transcription. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104726
Modern Language Association (MLA)
Abdel-Rahman, Wael M.…[et al.]. The Role of Chromosomal Instability and Epigenetics in Colorectal Cancers Lacking β-CateninTCF Regulated Transcription. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1104726
American Medical Association (AMA)
Abdel-Rahman, Wael M.& Lotsari-Salomaa, Johanna E.& Kaur, Sippy& Niskakoski, Anni& Knuutila, Sakari& Järvinen, Heikki…[et al.]. The Role of Chromosomal Instability and Epigenetics in Colorectal Cancers Lacking β-CateninTCF Regulated Transcription. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104726