Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α
Joint Authors
Zhang, Yan
Wang, Zhongqiu
Liu, Jun
Zhang, Zhenyu
Chen, Ye
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Syndecan-1 (SDC1), with a long variable ectodomain carrying heparan sulfate chains, participates in many steps of inflammatory responses.
But reports about the efforts of SDC1’s unshedding ectodomain on intestinal epithelial inflammation and the precise underlying mechanism are limited.
In our study, unshedding SDC1 from intestinal epithelial cell models was established by transfecting with unshedding SDC1 plasmid into the cell, respectively.
And the role of unshedding SDC1 in intestinal inflammation was further investigated.
We found that components of NF-κB pathway, including P65 and IκBα, and secretion of TNF-α were upregulated upon LPS stimulation in intestinal epithelial cells.
SDC1, especially through its unshed ectodomain, significantly lessened the upregulation extent.
It also functioned in inhibiting migration of neutrophils by downregulating secretion of CXCL-1.
Taken together, we conclude that suppressing SDC1 shedding from intestinal epithelial cells relieves severity of intestinal inflammation by inactivating NF-κB pathway and downregulating TNF-α expression.
These results indicate that the ectodomain of SDC1 might be the optional therapy for intestinal inflammation.
American Psychological Association (APA)
Zhang, Yan& Wang, Zhongqiu& Liu, Jun& Zhang, Zhenyu& Chen, Ye. 2016. Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104740
Modern Language Association (MLA)
Zhang, Yan…[et al.]. Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1104740
American Medical Association (AMA)
Zhang, Yan& Wang, Zhongqiu& Liu, Jun& Zhang, Zhenyu& Chen, Ye. Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104740