![](/images/graphics-bg.png)
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection
Joint Authors
Jarrett, Michael J.
Vázquez-Torres, Andres
Frank, Daniel N.
McCollister, Bruce D.
Henthorn, Patrick K.
Ir, Diana
Sheeder, Jeanelle
Guy, Michael S.
Anwar, Hiba Q.
Behbakht, Kian
Source
Infectious Diseases in Obstetrics and Gynecology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies.
We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff.
Methods.
Escherichia coli was incubated in phosphate-buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation.
Pelvic washings of the vaginal cuff were collected from hysterectomies occurring from June through October 2015.
In vitro techniques, 16S rRNA gene qPCR, and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff.
Results.
Mean bacterial colony formation in PBS was greater for E.
coli incubated in the presence of GTM (1.48 × 107 CFU/mL) versus without (9.95 × 105 CFU/mL) following 20-hour incubation (p=0.001).
Out of 61 pelvic washings samples, 3 were culture positive (≥5000 CFU/mL) with Enterococcus faecalis.
Conclusion.
In vitro experiments support a facilitating role of GTM on colony formation of E.
coli in PBS.
However, given the negative results of surgical site washings following adequate disinfection, the role of GTM in promoting posthysterectomy pelvic infections may be limited.
Analysis of pelvic washings revealed presence of E.
faecalis, but results were inconclusive.
Further studies are recommended.
American Psychological Association (APA)
Jarrett, Michael J.& Vázquez-Torres, Andres& Frank, Daniel N.& McCollister, Bruce D.& Henthorn, Patrick K.& Ir, Diana…[et al.]. 2016. Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection. Infectious Diseases in Obstetrics and Gynecology،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104863
Modern Language Association (MLA)
Jarrett, Michael J.…[et al.]. Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection. Infectious Diseases in Obstetrics and Gynecology No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104863
American Medical Association (AMA)
Jarrett, Michael J.& Vázquez-Torres, Andres& Frank, Daniel N.& McCollister, Bruce D.& Henthorn, Patrick K.& Ir, Diana…[et al.]. Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection. Infectious Diseases in Obstetrics and Gynecology. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104863