Viability Assessment of Genipa americana L. (Rubiaceae) Embryonic Axes after Cryopreservation Using In Vitro Culture
Joint Authors
Santos, Izulmé Rita Imaculada
Salomão, Antonieta Nassif
Source
International Journal of Agronomy
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-06
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Embryonic axes excised from seeds of Genipa americana L.
desiccated to different water contents were successfully cryopreserved by rapidly plunging seed samples directly into liquid nitrogen.
Control and cryopreserved embryonic axes were excised and grown in WPM culture medium for viability assessment.
All control embryonic axes (−LN2) excised from fully hydrated seeds (43.89% moisture content) germinated after 21 days of culture in vitro.
These high germination percentages persisted even after the water content of the seeds was as low as 6.79%.
After freezing in liquid nitrogen high germination percentages, 93%, 96%, and 93%, were observed for embryonic axes excised from seeds dehydrated to 13.26%, 9.57%, and 6.79 moisture content, respectively.
The cryopreservation technique described here is recommended for long term conservation of G.
americana germplasm.
American Psychological Association (APA)
Santos, Izulmé Rita Imaculada& Salomão, Antonieta Nassif. 2016. Viability Assessment of Genipa americana L. (Rubiaceae) Embryonic Axes after Cryopreservation Using In Vitro Culture. International Journal of Agronomy،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104912
Modern Language Association (MLA)
Santos, Izulmé Rita Imaculada& Salomão, Antonieta Nassif. Viability Assessment of Genipa americana L. (Rubiaceae) Embryonic Axes after Cryopreservation Using In Vitro Culture. International Journal of Agronomy No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104912
American Medical Association (AMA)
Santos, Izulmé Rita Imaculada& Salomão, Antonieta Nassif. Viability Assessment of Genipa americana L. (Rubiaceae) Embryonic Axes after Cryopreservation Using In Vitro Culture. International Journal of Agronomy. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104912
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104912