Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive
Joint Authors
Peng, Yangfeng
He, Quan Sophia
Cai, Jiang
Source
International Journal of Analytical Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Enantiomeric separation of citalopram (CIT) was developed using a reversed phase HPLC (RP-HPLC) with sulfobutylether-β-cyclodextrin (SBE-β-CD) as a chiral mobile phase additive.
The effects of the pH value of aqueous buffer, concentration of chiral additive, composition of mobile phase, and column temperature on the enantioseparation of CIT were investigated on the Hedera ODS-2 C18 column (250 mm × 4.6 mm × 5.0 um).
A satisfactory resolution was achieved at 25°C using a mobile phase consisting of a mixture of aqueous buffer (pH of 2.5, 5 mM sodium dihydrogen phosphate, and 12 mM SBE-β-CD), methanol, and acetonitrile with a volumetric ratio of 21 : 3 : 1 and flow rate of 1.0 mL/min.
This analytical method was evaluated by examining the precision (lower than 3.0%), linearity (regression coefficients close to 1), limit of detection (0.070 µg/mL for ( R )-CIT and 0.076 µg/mL for ( S )-CIT), and limit of quantitation (0.235 µg/mL for ( R )-CIT and 0.254 µg/mL for ( S )-CIT).
American Psychological Association (APA)
Peng, Yangfeng& He, Quan Sophia& Cai, Jiang. 2016. Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104927
Modern Language Association (MLA)
Peng, Yangfeng…[et al.]. Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1104927
American Medical Association (AMA)
Peng, Yangfeng& He, Quan Sophia& Cai, Jiang. Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1104927
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104927