Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing
Joint Authors
Shinbo, Kazunari
Kato, Keizo
Kaneko, Futao
Tangkawsakul, Wanida
Srikhirin, Toemsak
Baba, Akira
Source
International Journal of Analytical Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model.
The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR.
Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs.
The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor.
For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index.
We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test.
We obtained the lowest detection limits of 1.58 × 105 cells/ml for RBC-A and 3.83 × 105 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 108 cells/ml).
The surface of the biosensor can be efficiently regenerated using 20 mM NaOH.
In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing.
American Psychological Association (APA)
Tangkawsakul, Wanida& Srikhirin, Toemsak& Shinbo, Kazunari& Kato, Keizo& Kaneko, Futao& Baba, Akira. 2016. Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104929
Modern Language Association (MLA)
Tangkawsakul, Wanida…[et al.]. Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1104929
American Medical Association (AMA)
Tangkawsakul, Wanida& Srikhirin, Toemsak& Shinbo, Kazunari& Kato, Keizo& Kaneko, Futao& Baba, Akira. Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1104929
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104929