The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products
Joint Authors
Zhang, Dabing
Biswas, Sukumar
Fan, Wei
Li, Rong
Li, Sifan
Ping, Wenli
Li, Shujun
Naumova, Alexandra
Peelen, Tamara
Kok, Esther
Yuan, Zheng
Shi, Jianxin
Source
International Journal of Analytical Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Reliable methods are needed to detect the presence of tobacco components in tobacco products to effectively control smuggling and classify tariff and excise in tobacco industry to control illegal tobacco trade.
In this study, two sensitive and specific DNA based methods, one quantitative real-time PCR (qPCR) assay and the other loop-mediated isothermal amplification (LAMP) assay, were developed for the reliable and efficient detection of the presence of tobacco (Nicotiana tabacum) in various tobacco samples and commodities.
Both assays targeted the same sequence of the uridine 5′-monophosphate synthase (UMPS), and their specificities and sensitivities were determined with various plant materials.
Both qPCR and LAMP methods were reliable and accurate in the rapid detection of tobacco components in various practical samples, including customs samples, reconstituted tobacco samples, and locally purchased cigarettes, showing high potential for their application in tobacco identification, particularly in the special cases where the morphology or chemical compositions of tobacco have been disrupted.
Therefore, combining both methods would facilitate not only the detection of tobacco smuggling control, but also the detection of tariff classification and of excise.
American Psychological Association (APA)
Biswas, Sukumar& Fan, Wei& Li, Rong& Li, Sifan& Ping, Wenli& Li, Shujun…[et al.]. 2016. The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products. International Journal of Analytical Chemistry،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104942
Modern Language Association (MLA)
Biswas, Sukumar…[et al.]. The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products. International Journal of Analytical Chemistry No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1104942
American Medical Association (AMA)
Biswas, Sukumar& Fan, Wei& Li, Rong& Li, Sifan& Ping, Wenli& Li, Shujun…[et al.]. The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products. International Journal of Analytical Chemistry. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1104942
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104942