Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa
Joint Authors
Erasmus, R. T.
Matsha, T. E.
Pheiffer, Carmen
Humphries, Stephen E.
Gamieldien, Junaid
Kengne, Andre Pascal
Source
International Journal of Endocrinology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aims.
To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa.
Methods.
We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched.
Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc.
(Rockville, MD, USA).
Results.
Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia.
Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes.
Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes.
Conclusions.
Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes.
Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings.
American Psychological Association (APA)
Matsha, T. E.& Pheiffer, Carmen& Humphries, Stephen E.& Gamieldien, Junaid& Erasmus, R. T.& Kengne, Andre Pascal. 2016. Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa. International Journal of Endocrinology،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1105837
Modern Language Association (MLA)
Matsha, T. E.…[et al.]. Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa. International Journal of Endocrinology No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1105837
American Medical Association (AMA)
Matsha, T. E.& Pheiffer, Carmen& Humphries, Stephen E.& Gamieldien, Junaid& Erasmus, R. T.& Kengne, Andre Pascal. Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa. International Journal of Endocrinology. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1105837
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1105837