Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling
Joint Authors
Zhang, Chengshuo
Zhang, Jialin
Li, Le
Zhao, Bochao
Jiao, Ao
Li, Xin
Sun, Ning
Source
International Journal of Endocrinology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Glucocorticoid excess induces apoptosis of islet cells, which may result in diabetes.
In this study, we investigated the protective effect of ghrelin on dexamethasone-induced INS-1 cell apoptosis.
Our data showed that ghrelin (0.1 μM) inhibited dexamethasone-induced (0.1 μM) apoptosis of INS-1 cells and facilitated cell proliferation.
Moreover, ghrelin upregulated Bcl-2 expression, downregulated Bax expression, and decreased caspase-3 activity.
The protective effect of ghrelin against dexamethasone-induced INS-1 cell apoptosis was mediated via growth hormone secretagogue receptor 1a.
Further studies revealed that ghrelin increased ERK activation and decreased p38MAPK expression after dexamethasone treatment.
Ghrelin-mediated protection of dexamethasone-induced apoptosis of INS-1 cells was attenuated using the ERK inhibitor U0126 (10 μM), and cell viability increased using the p38MAPK inhibitor SB203580 (10 μM).
In conclusion, ghrelin could protect against dexamethasone-induced INS-1 cell apoptosis, at least partially via GHS-R1a and the signaling pathway of ERK and p38MAPK.
American Psychological Association (APA)
Zhang, Chengshuo& Li, Le& Zhao, Bochao& Jiao, Ao& Li, Xin& Sun, Ning…[et al.]. 2016. Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling. International Journal of Endocrinology،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1105878
Modern Language Association (MLA)
Zhang, Chengshuo…[et al.]. Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling. International Journal of Endocrinology No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1105878
American Medical Association (AMA)
Zhang, Chengshuo& Li, Le& Zhao, Bochao& Jiao, Ao& Li, Xin& Sun, Ning…[et al.]. Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling. International Journal of Endocrinology. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1105878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1105878
