The Roles of PI3KAKTmTOR and MAPKERK Signaling Pathways in Human Pheochromocytomas
Joint Authors
Zhang, Yushi
Tong, An-li
Wang, Fen
Cui, Yun-ying
Du, Juan
Li, Chunyan
Yan, Zhaoli
Source
International Journal of Endocrinology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objectives.
The roles of PI3K/AKT/mTOR and MAPK/ERK pathways involved in the pathogenesis of pheochromocytoma and paraganglioma (PPGL) were demonstrated mostly by in vitro studies with rat or mouse cells and were mainly studied at transcriptional level.
This study aimed to investigate the effect of these pathways on the proliferation of human PPGL cells and the activation of these pathways in PPGLs.
Methods.
Human PPGL cells were treated with sunitinib and inhibitors of PI3K (LY294002), MEK1/2 (U0126), and mTORC1/2 (AZD8055).
Cell proliferation was detected by MTT assay.
Protein phosphorylation was detected by Western blotting.
Results.
In most PPGLs, AKT, ERK1/2, and mTOR were activated.
LY294002 (10 μM), U0126 (10 μM), AZD8055 (1 μM), and sunitinib (1 μM) inhibited PPGL cell proliferation in ten primary cultures of tissues, including four from patients with gene mutations.
MEK1/2 inhibitor decreased mTOR phosphorylation.
Inhibition of mTOR reduced phosphorylation of AKT and ERK1/2.
Sunitinib inhibited phospho-ERK1/2 and phospho-mTOR.
Conclusion.
Our study suggested that PI3K/AKT/mTOR and MAPK/ERK signaling pathways play vital roles in human PPGL and are activated in most PPGLs.
Inhibiting multiple pathways might be a novel therapeutic approach for PPGLs.
American Psychological Association (APA)
Du, Juan& Tong, An-li& Wang, Fen& Cui, Yun-ying& Li, Chunyan& Zhang, Yushi…[et al.]. 2016. The Roles of PI3KAKTmTOR and MAPKERK Signaling Pathways in Human Pheochromocytomas. International Journal of Endocrinology،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1105916
Modern Language Association (MLA)
Du, Juan…[et al.]. The Roles of PI3KAKTmTOR and MAPKERK Signaling Pathways in Human Pheochromocytomas. International Journal of Endocrinology No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1105916
American Medical Association (AMA)
Du, Juan& Tong, An-li& Wang, Fen& Cui, Yun-ying& Li, Chunyan& Zhang, Yushi…[et al.]. The Roles of PI3KAKTmTOR and MAPKERK Signaling Pathways in Human Pheochromocytomas. International Journal of Endocrinology. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1105916
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1105916