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Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats
Joint Authors
Chang, Bai
Ye, Shoujiao
Song, Zhenqiang
Li, Jing
Li, Chunshen
Yang, Juhong
Source
International Journal of Endocrinology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In the study, type 2 diabetic rat model was established using streptozotocin (STZ) combined with a high-fat diet, and the rats were divided into control and diabetic groups.
Diabetic groups were further divided into nonintervening, simvastatin, Didang Decoction (DDD) early-phase intervening, DDD mid-phase intervening, and DDD late-phase intervening groups.
The expression level of MLCK was detected using Western Blot analysis, and the levels of cyclic adenosine monophosphate (cAMP), protein kinase C (PKC), and protein kinase A (PKA) were examined using Real Time PCR.
Under the electron microscope, the cells in the early-DDD-intervention group and the simvastatin group were significantly more continuous and compact than those in the diabetic group.
Compared with the control group, the expression of cAMP-1 and PKA was decreased in all diabetic groups, whereas the expression of MLCK and PKC was increased in early- and mid-phase DDD-intervening groups ( P < 0.05 ); compared with the late-phase DDD-intervening group, the expression of cAMP-1 and PKA was higher, but the level of MLCK and PKC was lower in early-phase DDD-intervening group ( P < 0.05 ).
In conclusion, the early use of DDD improves the permeability of vascular endothelial cells by regulating the MLCK signaling pathway.
American Psychological Association (APA)
Ye, Shoujiao& Song, Zhenqiang& Li, Jing& Li, Chunshen& Yang, Juhong& Chang, Bai. 2016. Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. International Journal of Endocrinology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1105955
Modern Language Association (MLA)
Ye, Shoujiao…[et al.]. Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. International Journal of Endocrinology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1105955
American Medical Association (AMA)
Ye, Shoujiao& Song, Zhenqiang& Li, Jing& Li, Chunshen& Yang, Juhong& Chang, Bai. Early Intervention of Didang Decoction on MLCK Signaling Pathways in Vascular Endothelial Cells of Type 2 Diabetic Rats. International Journal of Endocrinology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1105955
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1105955