Sexual Dimorphism in the Regulation of Estrogen, Progesterone, and Androgen Receptors by Sex Steroids in the Rat Airway Smooth Muscle Cells
Joint Authors
Campos-Lara, María Guadalupe
Zarazúa, Abraham
Ramírez-Vélez, Gabriela
Bazán-Perkins, Blanca
Guerra-Araiza, Christian
González-Arenas, Aliesha
Source
International Journal of Endocrinology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The role of sex hormones in lung is known.
The three main sex steroid receptors, estrogen, progesterone, and androgen, have not been sufficiently studied in airway smooth muscle cells (ASMC), and the sex hormone regulation on these receptors is unknown.
We examined the presence and regulation of sex hormone receptors in female and male rat ASMC by Western blotting and flow cytometry.
Gonadectomized rats were treated with 17β-estradiol, progesterone, 17β-estradiol + progesterone, or testosterone.
ASMC were enzymatically isolated from tracheas and bronchi.
The experiments were performed with double staining flow cytometry (anti-α-actin smooth muscle and antibodies to each hormone receptor).
ERα, ERβ, tPR, and AR were detected in females or males.
ERα was upregulated by E2 and T and downregulated by P4 in females; in males, ERα was downregulated by P4, E + P, and T.
ERβ was downregulated by each treatment in females, and only by E + P and T in males.
tPR was downregulated by P4, E + P, and T in females.
No hormonal regulation was observed in male receptors.
AR was downregulated in males treated with E + P and T.
We have shown the occurrence of sex hormone receptors in ASMC and their regulation by the sex hormones in female and male rats.
American Psychological Association (APA)
Zarazúa, Abraham& González-Arenas, Aliesha& Ramírez-Vélez, Gabriela & Bazán-Perkins, Blanca& Guerra-Araiza, Christian& Campos-Lara, María Guadalupe. 2016. Sexual Dimorphism in the Regulation of Estrogen, Progesterone, and Androgen Receptors by Sex Steroids in the Rat Airway Smooth Muscle Cells. International Journal of Endocrinology،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1106023
Modern Language Association (MLA)
Zarazúa, Abraham…[et al.]. Sexual Dimorphism in the Regulation of Estrogen, Progesterone, and Androgen Receptors by Sex Steroids in the Rat Airway Smooth Muscle Cells. International Journal of Endocrinology No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1106023
American Medical Association (AMA)
Zarazúa, Abraham& González-Arenas, Aliesha& Ramírez-Vélez, Gabriela & Bazán-Perkins, Blanca& Guerra-Araiza, Christian& Campos-Lara, María Guadalupe. Sexual Dimorphism in the Regulation of Estrogen, Progesterone, and Androgen Receptors by Sex Steroids in the Rat Airway Smooth Muscle Cells. International Journal of Endocrinology. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1106023
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106023